about the lab

M.U.T.A.N.T.   LAB
Miami University Transgenic Animal aNd Targeting Labhome.html
 

Institution: Miami University


Location: Oxford, OH


PI: Dr. Michael L. Robinson

Lab Manager: Brad Wagner


Research Field: Primarily lens development and bladder research.


Models: Primarily mouse and cell culture.

To understand what our lab is doing it is important to first understand the principles of lens structure and development.          

 

A specific portion of surface ectoderm begins to thicken in an area which overlies the neural ectoderm-derived optic vesicle. This thickening forms the lens placode, which will undergo a simultaneous invagination with the optic vesicle to form the lens vesicle and optic cup respectively. The lens vesicle is initially a hollow spherical ball of cells.  The cells closest to the optic cup, which will later become the retina, will differentiate from epithelial cells into fiber cells to form the basic structure of the mature lens(Robinson and Lovicu, 2004). Cells at the posterior of the lens vesicle elongate into primary fiber cells which fill the lumen of the vesicle. In this process the lens fiber cells loose their nuclei and the majority of their subcellelur organelles. The cells at the anterior of the lens remain epithelial cells, exhibit cuboidal morphology, and retain their ability to undergo mitosis. The lens continues to grow via the mitosis and subsequent differentiation of the lens epithelial cells into secondary fiber cells at slightly anterior to the lens equator.

Lens Development